Growth Inhibitory Effect of Resveratrol on Uterine Leiomyoma Cells.

Kang, Bo Young; Cho, Chi Heum; Kim, Sook Hyun; Shin, So Jin; Kwon, Sang Hoon; Park, Joon Cheol; Rhee, Jeong Ho; Kim, Jong In; Cha, Soon Do; Yoon, Sung Do

Original Article

Korean Journal of Obstetrics & Gynecology 2006;49(12):2597-2603.
Published online December 1, 2006.

Growth Inhibitory Effect of Resveratrol on Uterine Leiomyoma Cells.

Bo Young Kang, Chi Heum Cho, Sook Hyun Kim, So Jin Shin, Sang Hoon Kwon, Joon Cheol Park, Jeong Ho Rhee, Jong In Kim, Soon Do Cha, Sung Do Yoon

Department of Obstetrics and Gynecology, School of Medicine, Keimyung university, Daegu, Korea. ysd7505@dsmc.or.kr

Abstract

OBJECTIVE
To examine the effect of resveratrol on cell proliferation and cell cycle progression in the human uterine leiomyoma cells. METHODS: MTS [3-(4,5-dimethylthiazol-2-yl)5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] reduction assay was carried out to determine the viability of human uterine leiomyoma cells. Western blot analysis was done using anti pRB, anti-p21cip1/waf1, anti-p53, anti-cyclin E, anti CDK2 antibodies to detect the presence and expression of these proteins in treatment with resveratrol. DNA fragmentation assay was done to find the rate of apoptosis. Cell cycle analysis for resveratrol treated in human uterine leiomyoma cells was done by FACS (fluorescence-activated cell sorter) analysis. RESULTS: Resveratrol induced growth inhibition in a dose dependent manner, treatment with 100 ?M/L resveratrol blocked 30% cell growth. From Western blot analysis it revealed resveratrol induced the expression of p53 increasing. Caspase pathway was activated and cleavage of PARP was occurred. Apoptosis took place but in a reduced manner. FACS results showed that resveratrol increased the percentage of cells in sub G1 phase. CONCLUSION: Resveratrol, a dietry phytoalexin, inhibited cell proliferation and induced cell cycle arrest at sub G1 by enhancing the production of p53. These results indicate that resveratrol will be a promising agent chemopreventives or therapeutics against human uterine leiomyoma cells.

Key Words: Uterine leiomyoma cells, Resveratrol, Apoptosis