The Expression of p27kip1 and p57kip2 in Mouse Placenta During Gestation.

Kim, Sung Tae; Lee, Sung Ki; Kim, Kyong Jin; Gye, Myung Chan

Original Article

Korean Journal of Obstetrics & Gynecology 2003;46(10):1937-1942.
Published online October 1, 2003.

The Expression of p27kip1 and p57kip2 in Mouse Placenta During Gestation.

Sung Tae Kim, Sung Ki Lee, Kyong Jin Kim, Myung Chan Gye

¹Department of Obstetrics and Gynecology, Konyang University Hospital, Daejeon, Korea.
²Department of Biology, College of Natural Sciences, Kyonggi University, Suwon, Korea.
³Department of Life Science, College of Natural Sciences, Hanyang University, Seoul, Korea.

Abstract

OBJECTIVE
This study was to investigate the expression of CDK inhibitors, p27kip1 and p57kip2 during the growth and differentiation of mouse placenta. METHODS: Total RNA and protein were extracted from placenta of mouse sacrificed at day 12, 14, 16, 18 post-coitum (p.c.), then semi-quantitative RT-PCR and western blotting of p27kip1 and p57kip2 was carried out, respectively. RESULTS: p27kip1 mRNA was highly expressed in 18 days p.c. then other groups. But, p57kip2 mRNA expression was high in 12, 14, and 16 days p.c., then decreased in 18 days p.c. p27kip1 expression pattern was similar with mRNA. But, p57kip2 was higher in 14 days p.c. than other groups. CONCLUSION: This result shows that p27kip1 may play a role in late period of mouse placental development, and p57kip2 may play a role in middle period of mouse placental development.

Key Words: Mouse placenta, p27 kip1, p57 kip2