| Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines. |
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Jin Woo Kim, Mee Ran Kim, Jae Hoon Kim, Tae Eung Kim, Tae Chul Park, Hun Young Lee, Seung Jo Kim, Sung Eun Namkoong |
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| Abstract |
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Transforming growth factor-beta1(TGF-beta1) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-beta1 has been shown to inhibit growth and to rapidly reduce c-myc expression. However, the molecular mechanism of TGF-beta1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-beta1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines were incubated with varying concentration of TGF-beta 1, and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culturing in concentrations of 0.1~10 ng/ml in both cell lines. Northern blot analysis revealed c-myc mRNA expression was suppressed by 10 ng/ml of TGF-beta 1 following 6-hour of treatment in both cell lines. These results suggest that TGF-beta1 inhibits the growth of cervical carcinoma cells by suppressing c-myc oncogene expression. |
| Key Words:
Transforming growth factor-beta1, c-myc, Cervical cancer |
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