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Korean Journal of Obstetrics & Gynecology 2008;51(11):1269-1279.
Published online November 1, 2008.
Isolation and characterization of mesenchymal stem cells from human amnion and decidua.
Young Sun Yoon, Hyun Chul Jeong, Jong Ha Hwang, Hyun Jun Jee, Oye Sun Seok, Jung Youn Jo, Yun Joung Kim, Jae Kwan Lee
1Department of Obstetrics and Gynecology, Korea University College of Medicine, Research Center, Korea. jklee38@korea.ac.kr
2Women's Cancer Center Research Institute, Korea University College of Medicine, Research Center, Korea.
3RNL BIO CO., Ltd, Seoul, Seoul, Korea.
Abstract
OBJECTIVES
The purpose of this study is to isolate a population of multipotent cells from human amnion and decidua, respectively. METHODS: Human placentas (gestational age, 30~42 weeks) were obtained after vaginal or cesarean deliveries. Amnions and deciduas were divided mechanically. The collected cells from the amnion and decidua were cultured. Cultured cells were immunophenotypically characterized. The adipogenic, osteogenic and neurogenic differentiation capacities were tested, and their growth kinetics were analyzed. RESULTS: We successfully isolated MSCs from both the amnion and decidua. The phenotype of MSCs cultured from different fetal and maternal parts of the placenta was comparable. The growth kinetics of MSCs derived from amnions and deciduas were similar. Isolated MSCs were differentiated into various cell lines such as adipogenic, osteogenic, myogenic and neurogenic cells. CONCLUSIONS: The human amnion and decidua could be an excellent source of MSC because they are easily obtainable after delivery and showed a higher expansion capacity than that of MSCs from adult bone marrow.
Key Words: Amnion, decidua, mesenchymal stem cells


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