Expression of 14-3-3 proteins and Bax in the placenta of preeclampsia.

Park, Ji Kwon; Kim, Seon Im; Paik, Won Young

Original Article

Korean Journal of Obstetrics & Gynecology 2007;50(6):858-864.
Published online June 1, 2007.

Expression of 14-3-3 proteins and Bax in the placenta of preeclampsia.

Ji Kwon Park, Seon Im Kim, Won Young Paik

¹Department of Obstetrics and Gynecology, College of medicine, Gyeongsang National university, JinJu, Korea. wypaik@gnu.ac.kr
²Institute of Health Sciences, College of medicine, Gyeongsang National university, JinJu, Korea.

Abstract

OBJECTIVE
Purpose of our study was to examine the expression level of 14-3-3 proteins and Bcl-2 family and to estimate the interaction between 14-3-3 proteins and Bcl-2 family in normal and preeclamptic placenta. METHODS: Placental tissues were sampled from preeclampsia with caesarean delivery (n=5) and normal pregnant women with caesarean delivery (n=5). Western blot and immunoprecipitation related Western blotting were performed on all placental samples. Unpaired Student t-test was used to determine the statistical significance. RESULTS: Western blot analysis revealed that the expression of Bax and 14-3-3 zeta was significantly greater in the preeclamptic placenta than in the normal placenta. Immunoprecipitation related Western blotting revealed that the interaction between 14-3-3 zeta and Bax was significantly less in the preeclamptic placenta than in the normal placenta. CONCLUSION: Increased expression of Bax and reduced interaction (between) 14-3-3 zeta and Bax in preeclamptic placenta might influence pathogenesis or sequelae of preeclampsia. Further study is needed to identify the trigger that induces dissociation of Bax from 14-3-3 proteins.

Key Words: Preeclampsia, Placenta, 14-3-3 Proteins, Bax Protein