| Association of genetic polymorphisms in the peroxisome proliferator-activated receptor-gamma and methylenetetrahydrofolate reductase with preeclampsia in Korean women. |
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So Hyun Lee, Bo Hyun Park, Mi Hye Park, Hyesook Park, Sun Hee Chun, Jung Ja Ahn, Hyun Mee Ryu, Kwang Soo Lee, Hyun Young Park, Young Ju Kim |
1Department of Obstetrics and Gynecology, Ewha Medical Research Institute, College of Medicine, Ewha Womans' University, Seoul, Korea. kkyj@ewha.ac.kr
2Department of Preventive Medicine, Ewha Medical Research Institute, College of Medicine, Ewha Womans' University, Seoul, Korea.
3Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
4Division of Cardiovascular Diseases, Center for Biomedical Sciences, National Institute of Health, Seoul, Korea. |
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| Abstract |
OBJECTIVE
To investigate whether polymorphisms of genes encoding peroxisome proliferator-activated receptor-gamma (PPAR gamma) and methylenetetrahydrofolate reductase (MTHFR) are associated with preeclmapsia in Korean women and also to demonstrate whether there is any haplotypic association between preeclampsia and those genes. METHODS: DNA was extracted from whole blood of 226 preeclampsia patients and 235 healthy pregnant women. The genotypes of SNPs in PPAR gamma (-796A>G, P12A (C>G), H447H (161C>T)) and MTHFR (A222V (677C>T), E429A (1298A>C), R594Q (1793G>A)) were analyzed by a single base primer extension assay using a SNaPShot assay kit. Results were analyzed with the Student's t-test, Chi-square test, and Logistic regression analysis. Haplotype analyses were performed using Haploview 3.2 version. RESULTS: There were no significant differences in genotype or allele frequencies of PPAR gamma and MTHFR gene polymorphisms between preeclampsia patients and controls (p>0.05). No increase in the risk of preeclampsia for those genes was observed under any model of inheritance. Among SNPs of the PPAR gamma, MTHFR genes, only SNPs in MTHFR gene (677C>T, 1298A>C, 1793G>A) were in a strong linkage disequilibrium with each other (Lod score>2.0), but there were no significant differences in genotype distribution of haplotypes of MTHFR gene (TAG, CAG, CCA, CCG) between preeclampsia patients and controls (p>0.05). No statistically significant associations were observed between any haplotypes of MTHFR gene and preeclampsia risk. CONCLUSION: This study suggest that SNPs in PPAR gamma and MTHFR gene were not associated with preeclampsia in Korean women, and its haplotypes were also not associated with preeclampsia. |
| Key Words:
Preeclampsia, Single nucleotide polymorphism, PPAR gamma, MTHFR, Haplotypes |
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