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Korean Journal of Obstetrics & Gynecology 2006;49(9):1844-1854.
Published online September 1, 2006.
Comparison of placental endothelin-1 (ET-1) expression under hypoxic condition between normal versus preeclamptic pregnancy.
Yong Won Park, Ja Young Kwon, Young Han Kim, Yuri Kim, Jae Hak Lim, Muong Hwa Kang
1Department of Obstetrics and Gynecology, Yonsei University College of Medicine, Seoul, Korea. jaykwon@yumc.yonsei.ac.kr
2Institute of Women's Life Science, Yonsei University College of Medicine, Seoul, Korea.
Abstract
OBJECTIVE
To evaluate endothelin-1 (ET-1) expression in the villous explants from normal and preeclamptic (PE) placentae under hypoxic condition. METHODS: Villous explants from normal (n=5) and PE (n=4) placentae were obtained. To obtain hypoxic culture condition, villous explants were cultured in hypoxic chamber or treated with deferoxamine (DFO). ET-1 mRNA expressions in villous explants were evaluated by RT-PCR following 0, 24, and 48 h of culture in hypoxic chamber, and 0, 2, 4, 6 h following DFO treatment. ET-1 protein levels in media were measured by enzyme immunoassay. RESULTS: After 24 and 48 hours of incubation of villous explants from normal and PE placentae in hypoxic chamber, ET-1 mRNA and protein levels were increased in both groups, however, ET-1 production seemed to be more exaggerated in the villous explants from PE placentae. During 6 h of DFO exposure, ET-1 mRNA level was increased in the villous explants from PE placenta comparing to those from normal placentae (p<0.05). Interestingly, the increase of ET-1 mRNA expression in the villous explants from PE placentae was more exaggerated than those from normal placentae. Concordantly, increments of protein level between 0 to 2 h and 2 to 4 h were significantly higher in villous explants from PE placentae (p<0.05). CONCLUSION: ET-1 mRNA and protein were increased in villous explants from PE placentae compared to those from normal placentae under hypoxic condition. Furthermore, villous explants from PE placentae showed upregulated ET-1 expression upon hypoxic stimulation. This enhanced sensitivity to hypoxia may contribute to ET-1 overexpression in PE placenta in vivo and it needs further investigation for clarification.
Key Words: Preeclampsia, Endothelin-1, Hypoxia


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