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Korean Journal of Obstetrics & Gynecology 2005;48(5):1265-1270.
Published online May 1, 2005.
Effect of exogenous gonadotropin on HB-EGF expression in endometrium.
Seung Yup Ku, Byung Chul Jee, Chang Suk Suh, Seok Hyun Kim, Young Min Choi, Jung Gu Kim, Jin Yong Lee, Shin Yong Moon
1Department of Obstetrics and Gynecology, College of Medicine, Medical Research Center, Seoul National University, Korea. ymchoi@snu.ac.kr
2Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Korea.
3Department of Obstetrics and Gynecology, Eulji University School of Medicine, Korea.
Gonadotropin hyperstimulation for in vitro fertilization and embryo transfer (IVF-ET) has been considered to be harmful to the uterine receptivity. The role of HB-EGF has been implicated in the mouse embryonic implantation. In the present study, we assessed the effect of gonadotropin hyperstimulation on the expression of HB-EGF mRNA in murine endometrium. METHODS: Competitive, reverse transcription-PCR was performed to evaluate the expression of HB-EGF mRNA in the hyperstimulated murine endometrum from pregnant uteri of gestation day (g.d.) 5.5 after the injection of saline (placebo), pregnant mare serum gonadotropin (PMSG) 5 IU and 10 IU. RESULTS: HB-EGF expression was observed at 10-4-10-6 dilution concentrations of internal standard, and dual bands (423/360 bp) were observed at 10-5 dilution concentration. The expression of HB-EGF mRNA showed no significant change in the endometrial expression in PMSG groups, compared to the control group, on g.d. 5.5. There was no dose-response relationship between the two PMSG groups. CONCLUSION: Our data reveals that ovarian hyperstimulation using gonadotropin does not influence the expression of HB-EGF in the murine endometrium. Further studies are necessary to elucidate which stage of embryonic implantation and the expression of other substance is affected by the ovarian hyperstimulation.
Key Words: HB-EGF, Endometrium, Mouse, Ovarian hyperstimulation

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