Korean J Obstet Gynecol Search

CLOSE


Korean Journal of Obstetrics & Gynecology 2005;48(3):607-616.
Published online March 1, 2005.
Ex vivo expansion of umbilical cord blood-derived T-lymphocyte with autologous cord blood plasma.
Sang Soo Lee, Woo Seok Sohn, Han Moie Park, Young Man Kim, Jung Eun Mok
1Department of Obstetrics and Gynecology, University of Ulsan, Gangneung Asan Hospital, Gangneung, Korea.
2Department of Obstetrics and Gynecology, University of Ulsan, Seoul Asan Hospital, Seoul, Korea.jemok@amc.seoul.kr
Abstract
OBJECTIVE
The objective of this study was to establish a clinically applicable culture system by investigating the use of autologous cord blood plasma (ACBP) instead of fetal bovine serum (FBS) for the ex vivo expansion of umbilical cord blood (UCB) T-lymphocytes. METHODS: Fresh UCB mononuclear cell (MNC) fractions were isolated by Ficoll-Hypaque density centrifugation. The nonadherent MNC fractions were then cultured with the anti-CD3 antibody 5 microgram/mL plus IL-2 175 U/mL in the presence of 10% FBS, 10% ACBP or homologous cord blood plasma (HCBP). On day 8, proliferation rate, cell surface markers, cytotoxic assay of UCB T-lymphocytes according to the medium supplemented with FBS, ACBP or HCBP were evaluated. RESULTS: Proliferation studies demonstrated a significant increase in the proliferative ability of UCB T-lymphocytes incubated in anti- CD3 and IL-2 irrespective of the medium supplemented with FBS or ACBP. In the FBS supplemented medium, expressions of the activated T-lymphocytes were increased significantly after culture: CD3+CD8+, CD3+CD25+, CD3+CD38+, and CD45RO+ (p<0.05). Also in the ACBP supplemented medium, expressions of the activated T-lymphocytes were increased significantly after culture: CD3+ CD8+, CD3+CD25+, and CD45RO+ (p<0.05). In the HCBP supplemented medium, expressions of the activated T-lymphocytes were increased significantly after culture as in the ACBP: CD3+CD8+, CD3+CD25+, and CD45RO+ (p<0.05). Of the activated T-lymphocytes, increase of cytotoxic CD3+CD8+ cells increased significantly in the ACBP and HCBP groups compared to FBS group (p<0.05). CONCLUSION: These findings support the feasibility of ex vivo expansion of umbilical cord blood T-lymphocytes in the medium supplemented with autologous cord blood plasma, instead of fetal bovine serum, for future adoptive cellular immunotherapy.
Key Words: Umbilical cord blood, T-lymphocyte, Ex vivo expansion, Autologous cord blood plasma, Adoptive cellular immunotherapy
TOOLS
Share :
Facebook Twitter Linked In Google+ Line it
METRICS Graph View
  • 745 View
  • 10 Download
Related articles in Obstet Gynecol Sci

Correlations of Umbilical Venous Erythropoietin and Nucleated Erythrocytes with Blood Gases.1999 December;42(12)



ABOUT
ARTICLE & TOPICS
Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES
POLICY
FOR CONTRIBUTORS
Editorial Office
4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2266-7238    Fax: +82-2-3445-2440    E-mail: journal@ogscience.org                

Copyright © 2024 by Korean Society of Obstetrics and Gynecology.

Developed in M2PI

Close layer
prev next