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Korean Journal of Obstetrics & Gynecology 2004;47(6):1086-1092.
Published online June 1, 2004.
Cloning and Expression Pattern of FSTL1 mRNA in Normal Myometrium and Uterine Leiomyoma.
Yoon Sik Kim, Jae Sung Kim, Yong Ri Qian, Mei Hong Li, Ji Eun Kim, Yea Young Chun, Kwang Soo Kee, Hong Sung Cheon, Sung Jun Kim
1Department of Obstetrics and Gynecology, Kwangju Christian Hospital, Gwangju, Korea.
2Department of Cytogenetics Division, Kwangju Christian Hospital, Gwangju, Korea.
3Department of Genetic Science Graduate School of Chosun University, Gwangju, Korea.
Abstract
OBJECTIVE
To study the influence and cloning of differentially expressed genes in human female normal myometrium and uterine leiomyoma tissue. METHODS: In this experiment, human uterus tissues (n=25) were taken for total RNA isolation by using Trizol reagent. Differential display was performed by using GeneFishingTM DEG Kit and processed to cDNA sequencing and gene cloning for Follistatin-like 1 (FSTL1). Data were analyzed with the image Master VDS software and statistical significance was defined as p<0.05 by paired t test results. RESULTS: FSTL1 mRNA expression level was significantly higher (p<0.05) in normal and adjacent normal myometrium tissues than uterine leiomyoma tissue of women in the reproductive age. Whereas in the menopausal age, FSTL1 mRNA expression level was significantly higher (p<0.05) in uterine leiomyoma than normal myometrium. There was no significant differences between uterine leiomyoma and adjacent normal myometrium. CONCLUSION: Although the mechanisms of FSTL1 gene were uncertain, FSTL1 seemed to play an important role in the growth of uterine leiomyoma, it also might be related to the regulation of uterine leiomyoma growth inhibiting factors by modulating Follistatin related protein gene (FLRG) system.
Key Words: Follistatin-like 1 (FSTL1), Follistatin related protein gene (FLRG), Myometrium, Leiomyoma


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