| The Effects of Glutathione on Human Cancer Cells before and after the Application of Cisplatin. |
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Young Shin Cho, Hyoung Gyun Roh, Tae Gyu Ahn, Sei Jun Han |
1Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Kwangju, Korea.
2Department of Internal Medicine, College of Medicine, Chosun University, Kwangju, Korea. |
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| Abstract |
OBJECTIVE
The purpose of this study is to determine the time-dependant effects of Glutathione on the Cisplatin-induced cytotoxicity of human cervical carcinoma cells. METHODS: Two human cervical carcinoma cells, SiHa (squamous cell carcinoma cell), and CaSki (epidermoid metastatic carcinoma cell) were cultivated with RPMI1640 media. Reduced glutathione (GSH) and 2-oxo 4-thiazolidine carboxylic acid (OTC) were added one hour before and after Cisplatin (2-50 micro M/ml) was applied. The cells were incubated an additional 24 hours and viable cells were examined using a 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The MTT reduction rate of cisplatin-treated cervical carcinoma cells was increased significantly by the addition of glutathione (5 mM) or OTC (5 mM) both one hour before and after Cisplatin. A difference between MTT reduction rates one hour before and after cisplatin were not observed in either GSH or OTC. CONCLUSION: These results suggest that GSH and OTC have a protective effect on cisplatin-induced toxicity, and that this effect is about the same whether the agents were applied before or after the Cisplatin. |
| Key Words:
Cisplatin, Cervical carcinoma cell, Glutathione, OTC |
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