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Korean Journal of Obstetrics & Gynecology 2003;46(12):2410-2416.
Published online December 1, 2003.
Involvement of Oxidative Stress in Cisplatin-Induced Apoptosis in HeLa Cells.
Kyoung Hee Ko, Shin Ho Kim, Hae Joong Cho, Seong Hwan Oh, Heung Gon Kim, Rae Gil Park
1Department of Obstetrics and Gynecology, College of Medicine, Wonkwang University, Iksan, Korea.
2Department of Microbiology, College of Medicine, Wonkwang University, Iksan, Korea.
Abstract
OBJECTIVE
To determine whether oxidants are formed as part of the cisplatin-induced apoptotic process, intracellular markers of oxidative stress were examined. METHODS: Apoptotic death of HeLa cells by cisplatin was confirmed by flow cytometry. RESULTS: The pre-treatment with glutathione (GSH) significantly attenuated cisplatin-induced apoptosis through the reduction of reactive oxygen species (ROS) accumulation and diminished caspases-3 and 9 protease activity. Furthermore, z-VAD-fmk, an inhibitor of pan-caspase, effectively inhibited the activation of caspases and prevented apoptosis by cisplatin, although cisplatin-induced ROS generation was not attenuated. CONCLUSION: These data indicate that ROS may play a role as an upstream mediator of caspases. Taken together, our results suggest that oxidative stress mediates cisplatin-induced apoptosis in HeLa cells.
Key Words: Cisplatin, HeLa cells, Apoptosis


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