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Korean Journal of Obstetrics & Gynecology 2003;46(10):2028-2032.
Published online October 1, 2003.
Effects of Magnesium Sulfate on the Apoptosis and Invasion of Cytotrophoblasts under Hypoxic Condition.
Gui Se Ra Lee, Sa Jin Kim, Soo Young Hur, In Kwon, In Yang Park, Sang Hi Park, Jong Chul Shin, Soo Pyung Kim
1Department of Obstetrics and Gynecology, Catholic University Medical College, Seoul, Korea.
2Research Center for Clinical Medicine, Holy Family Hospital, Catholic University, Pucheon, Korea.
Abstract
OBJECTIVE
To determine the effect of magnesium sulfate (MgSO4) on the apoptosis and invasion of cytotrophoblasts in vitro under normal and hypoxic condition as assessed immunoblot analyses of Bcl-2/Bax, invasion assay and immunohistochemical staining of integrin alpha1. METHODS: Normal cytotrophoblasts were isolated from second trimester placentas and cultured in several physiologically relevant concentrations of MgSO4 under control tissue culture condition (20% O2) or hypoxic condition (1-2% O2). Apoptosis of cytotrophoblasts was estimated by immunoblotting for Bcl-2/Bax, invasiveness was estimated by invasion assay and immunohistochemical staining of Integrin alpha1. RESULTS: The expression of Bcl-2 did not change under standard condition, but it decreased under hypoxic condition with increasing of MgSO4 concentrations. The expression of Bax did not change under both standard condition and hypoxic condition with increasing MgSO4 concentrations. The invasiveness of cytotrophoblasts significantly decreased under both control and hypoxic conditions with increasing of MgSO4 concentrations. The expression of Integrin alpha1 immumohistochemical staining significantly decreased under control condition and showed decreasing tendency under hypoxic condition with increasing of MgSO4 concentrations. CONCLUSION: MgSO4 might induce cytotrophoblasts to the apoptosis and inhibit invasion of cytotrophoblasts under hypoxic condition.
Key Words: MgSO4, Cytotrophoblast, Bcl-2/Bax, Invasion, Integrin alpha1


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