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Korean Journal of Obstetrics & Gynecology 2003;46(2):259-267.
Published online February 1, 2003.
Study on the Apoptosis and its Mediators in the Placentas of Preclampsia using cDNA Expression Array.
Won Jun Choi, Wan Sung Choi, Jong Hak Lee, Won Young Paik
1Department of Obstetrics and Gynecology, College of Medicine, Gyeongsang National University, Chinju, Korea.
2Department of Anatomy, College of Medicine, Gyeongsang National University, Chinju, Korea.
Abstract
OBJECTIVE
The present study was designed to examine whether preeclampsia is associated with an increase in placental apoptosis and to detect differential expression of mediators involved in apoptosis. METHODS: Placental tissues from 10 cases of preeclampsia and 16 cases of control were analyzed using terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) staining. And cDNA expression array assay was performed for detecting mediators that were involved in placental apoptosis. RESULTS: In TUNEL staining, number of apoptotic nuclei were increased in placenta of preeclampsia. The expression of apoptotic signals was limited to trophoblasts. As a result of cDNA expression array assay, expressions of caspase-10, tumor necrosis factor receptor-1 (TNFR-1), and death domain receptor-3 (DDR-3) were increased and expression of cell division protein kinase-9 (CDK-9), cyclin A, insulin-like growth factor-1 (IGF-1), and insulin-like growth factor-binding protein-3 (IGFBP-3) were decreased in the placenta of preeclampsia. The expression of caspase-10, DDR-3, and IGFBP-3 between preeclampsia and normal revealed significantly different (p<0.05). CONCLUSION: Apoptosis was significantly increased in placenta of preeclampsia and various mediators were involved in apoptotic mechanism. Therefore, it was suggested that apoptosis might play a role in the pathophysiologic mechanisms of preeclampsia.
Key Words: Apoptosis, Peclampsia, Placenta, cDNA expression array
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