Factor V Gene Polymorphism with Preeclamptic Women in Korea.

Hong, Seung Hwa; Kim, Jeong Kyu; Choi, Jeong Weon; Ji, Ill Woon; Jeong, Eun Hwan; Kim, Hak Soon

Original Article

Korean Journal of Obstetrics & Gynecology 2002;45(11):1912-1916.
Published online November 1, 2002.

Factor V Gene Polymorphism with Preeclamptic Women in Korea.

Seung Hwa Hong, Jeong Kyu Kim, Jeong Weon Choi, Ill Woon Ji, Eun Hwan Jeong, Hak Soon Kim

Department of Obstetrics and Gynecology, Chungbuk National University College of Medicine, Cheongju, Korea.

Abstract

OBJECTIVE
This study was to evaluate the association of the G1691A mutation of factor V gene with preeclamptic patients in Korea. METHODS: Factor V genotype were determined in 113 preeclamptic patients and 100 normotensive pregnant women. A 267 base-pair DNA fragment of the factor V gene that includes nucleotide 1691 was amplified by PCR. PCR product was digested with MnlI restriction enzyme and the fragments were analysed by electrophoresis using 2% agarose gel. RESULTS: The difference of genetic and allelic frequencies between two groups could not be mentioned because all women were G1691G homozygotes. There was no individuals with mutant allele among preeclamptic patients and control women. CONCLUSION: There was no evidence of association between preeclampsia and factor V gene polymor- phism in Korean population.

Key Words: Preeclampsia, Factor V gene, Polymorphism