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Korean Journal of Obstetrics & Gynecology 2002;45(4):560-568.
Published online April 1, 2002.
Growth inhibition and induction of apoptosis in cervical cancer cell lines by green tea polyphenols.
Ying Min Jin, Sang Lyun Nam, Woong Sik Ahn
1Department of Oncology, Harbin Red Cross Central Hospital, Harbin, China.
2Chungnam National University Cancer Research Institutes, Chungnam National University, Taejon, Korea.
3Department of obstetrics and Gynecology, College of medicine, Chungnam National University, Taejon, Korea.
4Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Abstract
OBJECTIVE
The purpose of this article is to estimate the anti-cancer effects of the major components of the green tea (polyphenols, catechin and EGCG) and the mechanism of EGCG on different cervical cancer cell lines. METHODS: Six cervical cancer cell lines (HeLa, HeLaS3, Caski, SiHa, HT3 and C33A) were treated with 20 microgram/ml green tea polyphenols (GTPs), 50 micrometer catechin and various concentrations of (-)-epigallo- catechin-3-gallate (EGCG). The viabilities were determined by trypan blue exclusion assay, neutral red assay and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. DNA fragmentation and nuclear condensation were used to see whether EGCG-induced anti-proliferation effect was due to apoptosis. RESULTS: Both GTPs, catechin and EGCG had growth inhibition effects on cervical cancer cell lines, but EGCG appeared to be the most effective. What's more, the sensitivity of each cell lines to EGCG was different. HT3 cells (HPV negative, mutant type p53) were most sensitive to EGCG (estimate IC50: 10 micrometer). Caski (HPV-16 positive, wild type p53) and HeLaS3 cells (HPV-18 positive, wild type p53) were less sensitive (estimate IC50: 35 and 70 micrometer respectively). EGCG-induced apoptosis can be seen in all the cell lines and it happened as early as 8 hours after EGCG treatment. CONCLUSION: Green tea or EGCG alone will be beneficial to the cervical cancer patients.
Key Words: Green tea polyphenols, Cervical cancer, Apoptosis, Chemoprevention


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