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Korean Journal of Obstetrics & Gynecology 2001;44(8):1393-1400.
Published online August 1, 2001.
The Regulation of Cyclooxygenase-2 Expressionby Interleukin-1beta in WISH cells.
Young Jin Chang, Yoon Ki Park, Suk Whan Baek, Young Ki Lee, Dong Hyuk Lee, Hyun Woo Lee
Department of Obstetrics and Gynecology, Yeungnam University College of Medicine, Taegu, Korea.
Abstract
OBJECTIVE
To determine of the regulation of cyclooxygenase-2 (COX-2) expression by Interleukin-1beta in WISH cells. METHODS: Amnion WISH cells were incubated in media containing increasing concentrations of IL-1beta or with various inhibitors. Increased COX-2 expression was determined by Western blot analysis with anti-COX-2 antibody. Concomitant measurements of culture media PGE2 were made by an enzyme immunoassay. RESULTS: The COX-2 and prostaglandin E2 production induced by IL-1beta increased in a dose- and time-dependent manner. One of the regulating factors that induced COX-2 by IL-1beta was protein kinase C (PKC). PKC inhibitor, Ro 31-8220 was pretreated and continued treating by IL-1beta. Then, PKC inhibitor completely blocked COX-2 protein induction by IL-1beta. In contrast, COX-2 induction by IL-1beta after pretreating PKC stimulator, phobol 12-myristate 13-acetate was potentiated with synergism. Another factor in controlling COX-2 protein induction was identified as phosphatidylinositol 3-kinase (PI 3K). COX-2 protein induction by IL-1beta after pretreating PI 3K inhibitors, wortmannin and LY294002 strongly increased. This kind of result reflected that PI 3K act as negative regulator. COX-2 induction by IL-1beta was known to be regulated in not only transcription step, but also translation step after performing experiment of actinomycin and cycloheximide treatment. CONCLUSION: COX-2 protein and prostaglandin E2 production induced by IL-1beta were controlled by many factors in amnion cell. Among those factors, PKC and PI 3K have an important role, but their control mechanism act as positive and negative, respectively.
Key Words: WISH cell, Interleukin-1beta (IL-1beta), cyclooxygenase-2 (COX-2), protein kinase C (PKC), phosphatidylinositol 3-kinase (PI 3K)


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