The Mouse Embryo Culture System: Improving the Sensitivity for Use as a Quality Control Assay for Human in vitro Fertilization.

Song, J; Yang, N H; Kim, D S; Park, C S; Cho, S J

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Original Article

Korean Journal of Obstetrics & Gynecology 1998;41(2):489-493.
Published online January 1, 2001.

The Mouse Embryo Culture System: Improving the Sensitivity for Use as a Quality Control Assay for Human in vitro Fertilization.

J Song, N H Yang, D S Kim, C S Park, S J Cho

Abstract

OBJECTIVES
To determine whether the mouse embryo culture system can be sensitized to provide improved differentiation of suboptimal culture media for in vitro fertilization. DESIGN: Mouse embryo development in media prepared from one of two water sources were compared using embryos from two kinds of pretreatments, with either zona removal or intact. RESULTS: embryos with intact zona pellucida were able to develop in suboptimal culture conditions, even when cultured in tap water without protein sources. CONCLUSION: The mouse embryo culture system has deficiencies as an assay of culture medium quality, but the sensitivity of the assa.

Key Words: Mouse embryo culture, IVF

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