DNA Diagnosis or Classification of Hydatidiform Mole Using the Polymerase Chain Reaction.

Kim, S C; Chung, H W; Kim, H R

Original Article

Korean Journal of Obstetrics & Gynecology 1998;41(1):76-82.
Published online January 1, 2001.

DNA Diagnosis or Classification of Hydatidiform Mole Using the Polymerase Chain Reaction.

Abstract

OBJECTIVES
This study attempts to determine the genetic origin of hydatidiform moles, using the technique of Amplified Fragment Length Polymorphism[AMP-FLP] analysis which implies the polymerase chain reaction[PCR], and evaluate this method as a useful diagnostic method for genetic diagnosis or classification of hydatidiform moles. MATERIALS AND METHODS: A total of 11 cases which consists of 10 complete hydatidiform moles, and one partial hydatidiform mole were studied. In all cases of hydatidiform mole, deoxyribonucleic acid[DNA] was amplified from parental and molar samples by using primers for the variable number of tandem repeat[VNTR] alleles at D1S80 and for sex chromosome-specific sequences. RESULTS: Genetic origin was determined in all cases. All of the complete hydatidiform moles were androgenic, having only paternal alleles. Two of the complete hydatidiform moles were classified as dispermic on the basis of sex chromosome-specific sequences. But the partial hydatidiform mole revealed biparental contribution. CONCLUSIONS: Our results were compatible with the classical theory of androgenic origin of complete hydatidiform mole. And we also found partial hydatidiform mole has biparental origin. In conclusion, this PCR method was shown to be a easy and rapid method to diagnose and classify hydatidiform moles genetically.

Key Words: Hydatidiform moles, D1S80/VNTR, Polymerase chain reaction