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Korean Journal of Obstetrics & Gynecology 1997;40(11):2430-2436.
Published online January 1, 2001.
Comparison of the Expression of Transforming Growth Factor-beta in Microsurgical Reananstomosis of Rabbit Fallopian Tube.
C H Kim, S H Rho, E J Kim, K W Chung, D J Kwon, J H Kim, J W Lee, S P Kim
Department of Obstetrics and Gynecology, College of Medicine, Catholic University, Seoul, Korea.
Abstract
Twenty seven rabbits were divided into 3 groups of 1, 2-1 and 2-2(n=9 in each group). Arbitarilly cut ends of mid-ampullar portion of each tubes were microscopically reanastomosed using vicryl as suture material without intraluminal splint in group 1, using vicryl as suture material with intraluminal splint in group 2-1 and coagulation by Nd-YAG Laser beam as refusion method with intraluminal splint in group 2-2. A week after operation, the tubal tissue segments of reanastomosis were embedded into paraffin to examine the expression rate and intensity of TGF-beta1, 2 and 3 by immunohistochemical staining. The results were as follows. 1. The expression rate of TGF-beta1, 2 and 3 revealed no stastically significant difference between group 1 and 2. 2. Among comparisons group 2-1 and 2-2, the TGF-beta1 expression rate in group 2-2 showed lower value than that in group 2-1 with stastical significance(P<0.05). The above fact that tuboplasty with Nd-YAG Laser using intraluminal splint resulted in lower expression rate of TGF beta1 may suggest raising the succe ss rate of pregnancy.


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