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Korean Journal of Obstetrics & Gynecology 1997;40(1):154-160.
Published online January 1, 2001.
Inhibition of Cell Growth and Suppression of c-myc Gene Expression by Transforming Growth Factor-beta1 in Cervical Carcinoma Cell Lines.
Jin Woo Kim, Mee Ran Kim, Jae Hoon Kim, Tae Eung Kim, Tae Chul Park, Hun Young Lee, Seung Jo Kim, Sung Eun Namkoong
Abstract
Transforming growth factor-beta1(TGF-beta1) is known to be a potent growth inhibitor for many cell types, including most epithelial cells. In skin keratinocytes, TGF-beta1 has been shown to inhibit growth and to rapidly reduce c-myc expression. However, the molecular mechanism of TGF-beta1 action on cell growth of cervical carcinoma has not yet been elucidated. We thus assessed the effect of TGF-beta1 on the growth of cervical carcinoma cell lines. Two cervical squamous carcinoma cell lines were incubated with varying concentration of TGF-beta 1, and growth inhibition was evaluated with tetrazolium-based colorimetric assay. After culturing in concentrations of 0.1~10 ng/ml in both cell lines. Northern blot analysis revealed c-myc mRNA expression was suppressed by 10 ng/ml of TGF-beta 1 following 6-hour of treatment in both cell lines. These results suggest that TGF-beta1 inhibits the growth of cervical carcinoma cells by suppressing c-myc oncogene expression.
Key Words: Transforming growth factor-beta1, c-myc, Cervical cancer


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