The effect of udenafil (zydena) on the fertilization and early embryo development in mice.

Cheon, Keun Soo; Sim, Jae Chul; Yang, Hoe Saeng

doi:2011.54.9.508

Obstet Gynecol Sci > Volume 54(9); 2011 > Article

Original Article

Korean Journal of Obstetrics & Gynecology 2011;54(9):508-516.
DOI: https://doi.org/10.5468/KJOG.2011.54.9.508 Published online September 1, 2011.

The effect of udenafil (zydena) on the fertilization and early embryo development in mice.

Keun Soo Cheon, Jae Chul Sim, Hoe Saeng Yang

Department of Obstetrics and Gynecology, Dongguk University College of Medicine, Gyeongju, Korea. y31354@hotmail.com

Abstract

OBJECTIVE
To determine the effect of udenafil, a cyclic monophosphate-specific type 5 phosphodiesterase inhibitor, on fertilization and early embryo cleavage of mice. METHODS: This mammal study included randomly assigned male and female mice. The females were sacrificed after mating to evaluate their oocytes and embryos in four different time intervals following the treatment. Female mice were injected intraperitoneally with 5 IU of gonadotropin and human chorionic gonadotropin to stimulate follicular growth and induce ovulation. They were separately caged with males that had been gavaged with udenafil (0.06 mg/0.05 mL) and allowed to mate. After 24, 48, 72, and 96 hours of time intervals, females were killed, their oviducts were dissected out, and retrieved embryos were assessed for blastomere number and quality. Fertilization rates and numbers of embryos were evaluated after treatment. RESULTS: Fertilization rates were reduced in females that were mated with udenafil gavaged males. Over days 2-4, the numbers of embryo that of the treated group were significantly fewer than in the control group. There was also a tendency of impaired cleavage rates with those embryos. CONCLUSION: The impairments of fertility caused by udenafil have important implications for infertility centers and couples who are using this drug precoitally while attempting to conceive.

Key Words: Udenafil, Fertilization, Embryo cleavage