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Original Article
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Korean J Obstet Gynecol. 2010;53(2):127-136. Published online February 1, 2010.
DOI: https://doi.org/10.5468/kjog.2010.53.2.127
- Comparision in the yield of fetal nucleated red blood cell between the first-and second-trimester using double density gradient centrifugation.
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Yeon Jin Jeon, Kwon Hoon Kwon, Jong Won Kim, Myung Geol Pang, Sung Chul Jung, Young Ju Kim
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1Department of Biochemistry, Ewha Womans University, School of Medicine, Seoul, Korea.
2GenDix Inc., Seoul, Korea.
3Biomedlab Co., Seoul, Korea.
4Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Gyeonggi-Do, Korea.
5Department of Obstetrics and Gynecology, Ewha Womans University, School of Medicine, Seoul, Korea.
6Medical Research Institute, Ewha Womans University, Seoul, Korea. kkyj@ewha.ac.kr
- Abstract
- OBJECTIVE
The aim of our study was to make a practical comparative evaluation of the first and second trimesters in order to determine the period during which a higher yield of fetal nucleated red blood cells (FNRBCs) can be obtained. METHODS: NRBCs were isolated from maternal blood during the first and second trimesters of pregnancy using double Percoll gradients with different osmolarities. Magnetic activated cell sorting was performed with Kleihauer-Betke stain. We isolated fetal NRBCs from 10 mL of samples of maternal blood and determined fetal sex and fetal aneuploidy by fluorescence in situ hybridization (FISH). RESULTS: The average number of NRBCs was 9.85 in samples obtained during the first trimester and 14.88 in samples obtained during the second trimester (P=0.07). The average number of NRBCs with Y chromosome signals was 5.73 in the first trimester and 8.22 in second trimester (P=0.56). However, the percentage of NRBCs with Y chromosome signals in the first trimester (70.6%) was significantly higher than in the second trimester (59.8%) (P=0.049). We diagnosed the blood samples from 7 pregnant women having fetal aneuploidy using this method and the number of NRBCs was 18.4. CONCLUSION: The method using Percoll osmolarity and a double density gradient system may be a very useful method for separation of NRBCs in the first trimester of pregnancy and also in the second trimester.
Keywords :Fetal nucleated red blood cell;Fetal cell isolation;Magnetic activated cell sorting;Double density gradient system;Prenatal diagnosis
