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Original Article
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Korean J Obstet Gynecol. 2009;52(7):724-733. Published online July 1, 2009.
- Ovarian cancer related gene targeting with large circular antisense library.
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Kyung Oh Doh, Geun Soo Chun, Jae Chul Sim, Hoe Saeng Yang
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1Department of Obstetrics and Gynecology, College of Medicine Dongguk University, Gyeongju, Korea. y31354@hotmail.com
2Department of Physiology, Yeungnam University College of Medicine, Daegu, Korea.
- Abstract
- OBJECTIVE
The aim of this study is to find out the genes which are related to ovarian cancer cell growth using large circular antisense library. METHODS: Clones for antisense library were uni-directionally sub-cloned into pBS SK (-) vector. LC-antisense molecules were then purified from the culture supernatants of the bacterial competent cells superinfected with M13K07 helper bacteriophages. The LC-antisense library to 240 unigene clone was constructed and utilized in the identification of genes functionally involved in the growth of ovarian cancer cells. RESULTS: The 17 numbers out of the 240 numbers of the antisense library exerted a marked inhibitory effect on the growth of SK-OV 3. CONCLUSION: The putative functional categorization of each gene was then conducted via public databases. These candidates may be used as target genes for drug development or adjuvant of conventional chemotherapeutic drugs.
Keywords :Ovarian neoplasms;Large circular antisense DNA;Gene therapy
