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Original Article
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Korean J Obstet Gynecol. 2008;51(3):313-323. Published online March 1, 2008.
- The expression of annexin I and thymosin beta4 in cervical cancer.
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Yu Sun Lee, Hye Won Chung, Hye Sung Moon
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Department of Obstetrics and Gynecology, School of Medicine,Ewha Womans University, Seoul, Korea. mhsmhs@ewha.ac.kr
- Abstract
- OBJECTIVE
The aim of this study was to compare the expression of annexin I and thymosin beta4 in invasive cervical cancer including normal cervix and CIN. METHODS: In Ewha Womans University Mokdong Hospital, normal cervical tissues were obtained from healthy women (n=10), from patients with cervical intraepithelial neoplasia (CIN, n=10) and from patients with cervical cancer (n=33). The expressions of annexin I and thymosin beta4 mRNA and protein were examined by quantitative competitive-PCR and by western blot analysis. The expressions of annexin I and thymosin beta4 protein were measured by western blot analysis with thymosin beta4 peptides non treated and treated SiHa cells. RESULTS: The expression of thymosin beta4 mRNA and protein in cervical cancer were higher than that in normal cervix (p<0.05). The expression of annexin I mRNA and protein were higher than that in normal cervix and CIN (p<0.05). Thymosin beta4 and annexin I mRNA expressions were not significantly correlated with cervical cancer stage, or size of the tumor (p>0.05). But thymosin beta4 and annexin I protein expressions were increased according to the cancer stage. The expression of annexin I was slightly higher in thymosin beta4 treated SiHa cells than that in nontreated SiHa cells. CONCLUSIONS: Our results suggest that overexpression of thymosin beta4 and annexin I may play roles in progression of invasive cervical cancer. Thymosin beta4 upregulates expression of annexin I in invasive cervical cancer. Therefore, thymosin beta4 and annexin I may be biological markers in detecting the progression of invasive cervical cancer, and their interaction is important in invasive cervical cancer.
Keywords :Annexin I;Thymosin beta4;Cervical cancer
