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Original Article
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Korean J Obstet Gynecol. 2005;48(3):617-627. Published online March 1, 2005.
- Search for Gene Expression in Cervical Squamous Cell Carcinoma using GeneFishing(TM) DEG PCR Technique.
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Hee Jeong Yu, Byung Hoon Kim, Su Mi Bae, Young Wook Kim, Joon Mo Lee, Sung Eun Namkoong, Heung Jea Chun, Kye Hyun Nam, Young Lae Cho, Chong Kook Kim, Byoung Don Han, Woong Shick Ahn
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1Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea. hbk8392@yahoo.co.kr
2Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea.
3Department of Obstetrics and Gynecology, College of Medicine, Soonchunhyang University, Bucheon, Korea.
4Department of Obstetrics and Gynecology, College of Medicine, Kyungbook National University, Taegu, Korea.
5Department of College of Pharmacy, Seoul National University, Korea.
6BMS Korea Research Center, Seoul, Korea.
- Abstract
- OBJECTIVE
The aim of this study was to investigate the gene expression profiles using GeneFishing(TM) DEG kit in Korean women with cervical squamous cell carcinoma. METHODS: Cervical cancer biopsies were obtained from patients at the Department of Obstetrics and Gynecology, St. Mary's hodpital. In this study, we used a common reference that was mixed with an equal amount of RNA extracted from non-cervical cancer patients. The profiles of expression genes between cervical normal and squamous cell carcinoma tissue were identified using GeneFishing(TM) DEG Kit and screened by BLAST search. RESULTS: Almost 100 differential expressed genes were identified in universal control and cervical squamous cell carcinoma, 53 of differential expressed genes, up-regulated expression of 32 and 21 down-regulated expression was sequenced. Up-regulated genes were calcylin, calgranulin A, TRK oncogene, HLC5, fibrillarin, collagene type I alpha1 etc. and down-regulated genes were galectin 1, PRP8 pre-mRNA precessing factor 8 homology, clusterin etc. CONCLUSION: We identified gene expression profile in cervical squamous cell carcinoma using GeneFishing(TM) Kit in Korean women. The functional genomics of these genes should be further studied.
Keywords :Cervical squamous cell carcinoma;GeneFishing;Differential expressed genes;Human papilloma virus
