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Original Article
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Korean J Obstet Gynecol. 2001;44(9):1602-1608. Published online September 1, 2001.
- Damage of Intracytoplasmic Meiotic Spindles in Cryopreserved-Thawed Mouse Oocytes.
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Seok Hyun Kim, Sung Mi Choi, Byung Chul Jee, Hee Sun Kim, Sun Kyung Oh, Chang Suk Suh, Young Min Choi, Jung Gu Kim, Jin Yong Lee, Shin Yong Moon
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1Department of Obstetrics and Gynecology, College of Medicine, Seoul National University, Seoul, Korea.
2Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Korea.
- Abstract
- OBJECTIVE
To investigate the extent of meiotic spindle damages in cryopreserved-thawed mouse mature oocytes. METHODS: After slow freezing and ultra-rapid thawing using 1.5M dimethylsulfoxide(DMSO), mouse mature oocytes were stained by anti-alpha tubulin monoclonal antibody. The meiotic spindle and chromosomes configuration were assessed using confocal microscope. The influence of time to post-hCG oocytes retrieval (i.e. 12 hrs vs 17 hrs) was also evaluated. RESULTS: The normal meiotic spindles were observed in 89.8% of post-hCG 12 hrs group, and 80.1% of post-hCG 17 hrs group, and these were significantly lower than that of each unfreezed control. Post-hCG 12 hrs group showed a significantly higher incidence of normal meiotic spindles, compared with post-hCG 17 hrs group. CONCLUSION: The extent of meiotic spindle damages was significantly increased after cryopreservation in mouse mature oocytes. We proposed that 12 hrs interval of post-hCG oocytes retrieval may be more beneficial.
Keywords :Mouse oocytes;Cryopreservation;DMSO;Slow freezing and ultrarapid thawing;Meiotic spindle
