| Clinical Efficacy of HPV DNA Chip test in Human papillomavirus detection of Uterine Cervix. |
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Je Hoon Lee, Ki Heon Lee, In Ho Lee, Hyuk Jun Woo, Seok Ju Seong, Tae Jin Kim, Kyung Taek Lim, Jae Uk Shim, Chong Taik Park |
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Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Sungkyunkwan University School of Medicine, Seoul, Korea. 1103khl@hanmail.net |
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| Abstract |
OBJECTIVE
This study was performed to investigate the efficacy of HPV DNA chip method for detection and genotyping of various human papillomavirus in the patients with intraepithelial lesions of uterine cervix. METHODS: The study subjects included two hundred patients with abnormal Pap smear from July 2004 to October 2004. After confirmed the pathological status of the cervix with colposcopic biopsy or conization, we evaluated for HPV infection and genotyping with the commercially available Hybrid-Capture II assay (HC-II) and HPV DNA chip. Then we compared the concordance rate between the two methods for the detection of HPV and analysed the HPV genotypes. RESULTS: We compared the results in HPV DNA chip with those in HC-II. In result, the concordance rate between the two methods for the detection of HPV was 85.5% (171 of 200 cases). In 111 patients confirmed the presence of lesions higher than flat condyloma in cervix by pathologc examination, sensitivities of HC-II and HPV DNA chip in detecting HPV were 91.0% and 88.3%, respectively. In HPV DNA chip, HPV-16 was the most frequent type (14.7%) in all patients, the next frequent types were HPV-58 (14.1%) and HPV-18 (9.2%). CONCLUSION: We confirmed that HPV DNA chip method was as sensitive and effective method for detecting HPV in cervical lesions as HC-II. And that it would provide useful clinical information on genotyping and multiple infections of HPV. |
| Key Words:
Abnormal pap smear, Cervical cancer, Cervical precancerous lesion, HPV, HC-II, HPV DNA chip |
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