Clusterin confers paclitaxel resistance in ovarian cancer.

Park, Dong Choon; Yeo, Seung Geun; Mok, Samuel C

Search: Korean J Obstet Gynecol Search

CLOSE

Obstet Gynecol Sci > Volume 48(10); 2005 > Article

Original Article

Korean Journal of Obstetrics & Gynecology 2005;48(10):2313-2320.
Published online: October 1, 2005

Clusterin confers paclitaxel resistance in ovarian cancer.

Dong Choon Park, Seung Geun Yeo, Samuel C Mok

¹Department of Obstetrics and Gynecology, Saint Vincent Hospital, The Catholic University of Korea.
²Department of Otolaryngology, College of Medicine, Kyung Hee University, Korea.
³Department of Obstetrics, Gynecology and Reproductive Biology, Division of Gynecologic Oncology, Brigham and Women's Hospital, Harvard Medical School, MS, U.S.A.

Abstract

OBJECTIVE
To evaluated whether clusterin over-expression is significantly correlated with paclitaxel resistance in ovarian cancer cell lines. METHODS: Clusterin was validated by performing expression profiling analysis and subsequently, the correlation between clusterin mRNA expression levels and the IC50 of paclitaxel was tested. Transfection of clusterin was performed on SKOV3, which expressed paclitaxel-sensitivity and low level of clusterin, and transfection of clusterin siRNA on PEOH, which expressed paclitaxel-resistance and high level of clusterin, to evaluate their effect on chemo-sensitivity, apoptosis, and cell cycle by XTT assay, cell death ELISA, and flow cytometry, respectively. RESULTS: Clusterin mRNA and protein expression levels were significantly correlated with paclitaxel resistance (P<0.001). Transfection of cluterin on SKOV3 significantly decreased apoptosis and increased paclitaxel resistance. And transfection of clusterin siRNA on PEOH significantly increased paclitaxel-sensitivity (P<0.05), and shifted cells from S to G2/M phase of the cell cycle after paclitaxel treatment. CONCLUSION: These findings suggested that clusterin overexpression confers paclitaxel-resistance by the modulation of the apoptotic pathway and cell cycle progression in ovarian cancer cells.

Keywords: Clusterin; Ovarian cancer; Paclitaxel-sensitivity and resistance

TOOLS

Full text via DOI
Download Citation

Share :

METRICS

1,579 View
10 Download

Related articles in Obstet Gynecol Sci

Intraperitoneal cisplatin chemotherapy for advanced ovarian cancer.1993 October;36(10)

A Case of Uterine Serosal Pregnancy with Both Ovarian Cysts.2005 March;48(3)

Clusterin expression and paclitaxel resistance in cervical cancer cell lines.2006 May;49(5)

The Role of Chemotherapy Sensitivity and Resistance Assays in Ovarian Cancer.2006 August;49(8)

Solitary mesenteric recurrence of ovarian cancer.2007 August;50(8)

ABOUT

ARTICLE & TOPICS

Article category

Browse all articles >

Topics

Browse all articles >

BROWSE ARTICLES

POLICY

FOR CONTRIBUTORS

Editorial Office: 4th Floor, 36 Gangnam-daero 132-gil, Gangnam-gu, Seoul 06044, Korea.
Tel: +82-2-2271-6788 Fax: +82-2-3445-2440 E-mail: journal@ogscience.org