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Korean Journal of Obstetrics & Gynecology 2005;48(5):1271-1281.
Published online May 1, 2005.
Effects of Vero Cells Co-culture System on The In Vitro Development of Mouse Preimplantation Embryos in Media with Different Composition of Glucose and Pyruvate.
Ju Hwan Kim, Ki Hwan Lee, Song Kyong Son, Hai Bum Song, Kil Chun Kang
1Department of Obstetrics and Gynecology, College of Medicine, Chungnam National University, Korea. kckang@cnuh.co.kr
2Infertility Clinic, Department of Obstetrics and Gynecology, Chungnam National University Hospital, Korea.
3Department of Life Resources, Daegu University, Korea.
Abstract
OBJECTIVE
The purpose of this study was to examine the effects of vero cells co-culture system on the in vitro development of mouse preimplantation embryos in culture media with different composition of glucose and pyruvate. METHODS: Two-cell embryos were collected from 4-5 weeks old ICR mice. Seven hundreds twenty two embryos were cultured with or without vero cells monolayer in four media with different compositions that was manufactured by two DMEM media with (DMEM-GGP) or without (DMEM-G) glucose and pyruvate. In control, DMEM-G medium which is currently using for human embryo culture in our infertility clinic was used. Group I (DMEM-G(1/4)GP) was cultured in medium which was mixed three volume of DMEM-G and one volume of DMEM-GGP, and group II (DMEM-G(1/2)GP) was cultured in medium which was mixed same volume of DMEM-G and DMEM-GGP, and group III was cultured in DMEM-GGP. All media were added to 20% hFF. Results between different groups were analyzed using a Chi-square test, and considered statistically significant when p value was less than 0.05. RESULTS: The developmental rate into 3-cell
Key Words: Co-culture, Mouse preimplantation embryo, Vero cells, DMEM


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