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Korean Journal of Obstetrics & Gynecology 2003;46(1):28-33.
Published online January 1, 2003.
Comparative Analysis of Multiplex PCR and Hybrid Capture System in the Detecttion of Human Papillomavirus in the Uterine Cervix.
Sei Jun Han, San Kim, Min Chang Kang, Ju Seong Lim, Tae Gyu Ahn, Byung Rai Lee
1Department of Obstetrics and Gynecology, College of Medicine, Chosun University, Gwangju, Korea.
2Department of Biochemistry, College of Medicine, Chosun University, Gwangju, Korea.
Abstract
OBJECTIVE
To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples. METHODS: This study determined the HPV infection and its risk group (subtype) in uterine cervical swab samples of 91 Korean women by HPV hybrid capture I and multiplex-PCR method. RESULTS: The prevalence of HPV infection determined by muliplex-PCR was more higher than by hybrid capture method I. Of 65 women with nonspecific uterine cervical lesions, the frequence of HPV-positivity was 14/65 (12 low-risk and 2 high-risk HPV) by hybrid capture method I and was 37/65 (34 low-risk and 3 high- risk HPV) by multiplex-PCR method, respectively. The frequence of high-risk HPV was 2/6 in LSIL, 7/14 in HSIL, 4/6 in uterine cervical cancer by hybrid capture method I, and was 2/6 in LSIL, 8/14 in HSIL, 4/6 in uterine cervical cancer by multiplex-PCR method. CONCLUSION: These results suggest that multiplex-PCR is more sensitive, simple and cost-effective technique than Hybrid-capture I in the detection of HPV infection in the uterine cervix.
Key Words: HPV, Multiplex PCR, Hybrid capture I


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