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Korean Journal of Obstetrics & Gynecology 2001;44(9):1678-1684.
Published online September 1, 2001.
The Effect of Exocelomic Fluid in the First Trimester Pregnancy on Trophoblast Cell Proliferation in Vitro.
Bum Chae Choi, Jeong Wook Kim, Ji Ae Lee, Keun Jai Yoo, Hye Kyung Byun, Chang Sun Hwang, In Kul Moon, Soo Nyung Kim, Kwang Hyung Baek
1Center for Recurrent Miscarriage and Infertility, Creation and Love Women's Hospital, Kwangju, Korea, SungKyunKwan University, Seoul, Korea.
2Department of Obstetrics and Gynecology, Infertility Research Laboratory, SungKyunKwan University, Seoul, Korea.
3Endocrinology laboratory, School of Medicine, SungKyunKwan University, Seoul, Korea.
4Department of Obstetrics and Gynecology, College of Medicine, Konkuk University, Seoul, Korea.
5Department of Microbiology, College of Medicine, Pochon CHA University, Seoul, Korea.
Abstract
OBJECTIVE
To investigate the effect of exocelomic fluid in first trimester pregnancy on trophoblast cell proliferation in vitro. METHODS: The coelomic fluid was obtained from women with apparently normal pregnancies (n=9) and women presenting with missed abortion (n=22). The concentrations of cytokines in coelomic fluid were determined by two steps sandwich ELISA. The detection limits were the 4 pg/ml for IFN-gamma, 1 pg/ml for TNF-alpha, 2 pg/ml for IL-6 and 5 pg/ml for IL-10, respectively. The data are presented as mean+/-SEM. Statistical analysis was performed by Mann-Whitney U test. Trophoblast cell (Jeg-3 choriocarcinoma cell line) proliferation in vitro was determined using colorimetric immunoassay, based on the measurement of BrdU incorporation using DNA synthesis. The optical absorbance of the samples at 450 nm was measured using an ELISA reader. The data are presented as absorbance in the samples (mean+/-SEM). Statistical analysis was performed using regression analysis and t-test. RESULS: Th-2 type cytokines are present to some extent and IL-6, one of Th-1 type cytokines, also exists in the coelomic fluid from the missed abortion. Coelomic fluids from the majority of normal pregnancies inhibited trophoblast proliferation in vitro significantly higher than fluids from the missed abortion. CONCLUSION: These data showed that exocoelomic fluids may have a unique immune privilege surrounding developing embryo in the early pregnancy. Further studies are required to determine the goowth factors in coelomic fluids from normal pregnancies and missed abortion, and to evaluate the influence on the development of early pregnancy complications.
Key Words: Cytokine, Exocelomic fluid, Recurrent spontaneous abortion, Trophoblast
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