Fetal Cells in Maternal Blood: More Efficacious FISH Analysis by Using GPA to Identify Fetal cells.

Jee, K J; Kim, S C; Lee, S S; Moon, Y G

Original Article

Korean Journal of Obstetrics & Gynecology 1998;41(5):1346-1351.
Published online January 1, 2001.

Fetal Cells in Maternal Blood: More Efficacious FISH Analysis by Using GPA to Identify Fetal cells.

Abstract

The possibility of prenatal genetic screening using fetal cells isolated from maternal blood has been reported. However, it is essential to recognize of real fetal nRBC for an accurate prenatal diagnosis because the number of fetal cells in maternal circulation is very rare and some maternal cells can be contaminated in it, in spite of enrichment of maternal pheriperal blood. Therefore, we attempted the method called ""Immuno-FISH"" that GPA (glycophorin A) conjugated to cytoplasmic membrane and a DNA probe tagging fluorescene with binding to nucleus, simultaneously, for more efficacious analysis of fetal genetic screening. As the results of evaluated from 42 pregnancy women; we could identify 8.2 fetal nRBCs from 20 ml maternal pheriperal blood, we can observe the signal of Immuno-FISH onto nRBC within 16 on the 42 samples (35.6%), successfully, and we could have more efficacious results as the sensitivity was 71.74%, the specificity was 88.8%.

Key Words: Fetal cells in maternal blood, Immuno-FISH, GPA