In Vitro Culture of Human Endometrial and Cytotrophoblast Cells.

Lee, Bo Yon; Kim, Seung Bo

Original Article

Korean Journal of Obstetrics & Gynecology 1997;40(6):1171-1177.
Published online January 1, 2001.

In Vitro Culture of Human Endometrial and Cytotrophoblast Cells.

Bo Yon Lee, Seung Bo Kim

Department of Obstetrics and Gynecology, KyungHee University, College of Medicine, Korea.

Abstract

Implantation is a cascade of process which fertilized conceptal tissue move into maternalendometrium. Recently, with development of life science, the informations about humanimplantation process have been important topic with regard to the reproductive medicaltechnology. However, the precise mechanism and the factors involved in the process is notclearly understood, because the human experiments have been inhibited due to ethical andreligious views to the point. To study the human implantation process, we develop the modelto culture the human endometrium and trophoblast in vitro. The microscopic findings ofcultured monolayer of endometrial glandular epithelium and stroma in respond to co-culturedtrophoblast cells were observed. As results, trophoblasts didn't change the microscopicmorphology of endometrial glandular layer. However, trophoblast cells destructed the higherconcentration of epidermal growth factor in culture media. These results suggests thatepidermal growth factor may be a important factor for trophoblast invasion to stromal layerand trophoblast may invade the glandular layer by different mechanism to endometrial stroma.

Key Words: Implantation, Trophoblast, Cytotrophoblast, Endometrium, In vitro